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. 2012 Nov 14;7(11):e49579. doi: 10.1371/journal.pone.0049579

Figure 4. Survival after exposure to ionizing radiation.

Figure 4

A–B) Clonogenic survival. Cells were treated with 10 nM (ZHER2∶342)2 for 2 hours prior to irradiation and allowed to recover for 16 hours before reseeding. Mean values and standard deviations are calculated on at least 7 replicates. The linear quadratic model was used for curve fitting and an unpaired t-test to test for significance. A) SKBR-3. Inset; after irradiation with 8 Gy the cells treated with (ZHER2∶342)2 had a significantly lower (P<0.01) survival than the control group. B) MCF-7 cells. Inset; after irradiation with 8 Gy the cells treated with (ZHER2∶342)2 did not differ from the control group. C) Growth extrapolation method. SKBR-3 cells were treated with 16.6 nM (ZHER2∶342)2, ZHER2∶342 or left untreated for two hours, irradiated and then reseeded. The figure shows the number of cells normalized to the starting value in each group after 28 days of cultivation in normal cell culture medium.