Figure 10. Accumulation of superoxide anion and hydrogen peroxide in rice panicle under heat shock.

Plants at the reproductive stage were treated at 40°C for 8 h, and the florets of young panicles were collected at 0 min (as a control), 20 min, 60 min, 2 h, 4 h and 8 h. Immediately following heat-treatment, the florets were treated with NBT solution to detect superoxide anion or DAB solution to detect hydrogen peroxide in the dark for 3 h and 24 h, respectively.