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. 2012 Nov 14;7(11):e48656. doi: 10.1371/journal.pone.0048656

Figure 2. Expression of chondrogenic and adipogenic markers during monolayer culture of wt and DN-Erg chondrocytes.

Figure 2

A. Expression of chondrogenic genes Col2a1, Sox9, Col10, and Runx2. Gene expression was evaluated by RT-qPCR. B. Expression of adipogenic genes Adpn, and Pparγ. Gene expression was evaluated by RT-qPCR. The reported target gene: Hprt transcript ratio in chondrocytes was normalised to the target gene:Hprt transcript ratio (set to 1) of freshly plated wt chondrocytes (day 0). Data represent the mean of at least 2 independent chondrocyte cultures from 2 distinct mice for each genotype.