Table 2. Correlation of microarray data and RT-qPCR analysis.
| Microarray | Real-Time qPCR | ||||
| (Fold-change between day 20 vs day 0 of culture) | (Fold-change between day 20 vs day 0 of culture) | ||||
| Sequence Name | Accession | WT | DN-ERG | WT | DN-ERG |
| Cartilage Development | |||||
| Scinderin | NM_009132 | −2 | −71 | −14 | −795 |
| Matn3 | NM_010770 | −7 | −39 | 1 | −2 |
| Metalloproteinases | |||||
| ADAMTS5 | NM_011782 | 2 | 49 | 4 | 56 |
| MMP3 | NM_010809 | 40 | 284 | 13 | 87 |
| MMP9 | NM_013599 | −1 | 26 | 1 | 137 |
| MMP13 | NM_008607 | 11 | 49 | 10 | 71 |
| Cathepsine | |||||
| CTS C | NM_009982 | 4 | 131 | 39 | 317 |
| CTS S | NM_021281 | 6 | 288 | 52 | 1116 |
| Adipocyte Differentiation | |||||
| Plin | NM_175640 | 4 | 64 | 3 | 16 |
| FABP4 | NM_024406 | 3 | 16 | 146 | 284 |
| Adipoq | NM_009605 | 9 | 84 | 200 | 467 |
Comparison of fold change in the expression of 11 genes implicated in chondrogenesis or adipogenesis as determined by microarray analysis and RT-qPCR.
Fold change between day 0 and day 20 observed by microarray-analysis and RT-qPCR for wt and DN-Erg chondrocytes are shown. Expression levels of target genes obtained by RT-qPCR were normalised to Hprt. Upregulation is indicated by positive values and downregulation is indicated by negative values.