Figure 2. Methylation levels across 39 CpG sites in exon 1F for each subject.

Methylation level of GR exon 1F at individual CpG sites for females (n = 46) (2A) and males (n = 46) (2B). Each point in the figure represents a single individual's percentage methylation value for each of the 39 CpG sites that were analyzed by bisulfite pyrosequencing. The points were color coded by the graph option in Excel and each color and shape represents an individual subject. The graph illustrates the marked individual differences (indicated by the shape and color of markers) in percentage methylation at each CpG site. Figure (2C) presents the promoter sequence of exon 1F showing the CpG sites and their position. Highlighted regions represent known or putative canonical (italics) and noncanonical (not italics) NGFI-A–binding sites, with the shaded grey area indicating the beginning of the exon (following McGowan et al (23)).