Table 1. Bacterial strains and plasmids used in this study.
| Strain or plasmid | Descriptiona | Reference |
| Acinetobacter sp. | ||
| Tol 5 | Wild type strain | [20] |
| Tol 5 T1 | Tol 5 mutant lacking adhesive and autoagglutinating properties | [22] |
| ΔataA | Tol 5 mutant with defect in ataA by allelic marker exchange | This study |
| ADP1 | A representative strain of Acinetobacter | [33] |
| E. coli | ||
| DH5α | Host for routine cloning | Takara |
| DH10B | Host for routine cloning | Invitorogen |
| S17-1 | Donor strain for conjugation | [45] |
| BL21 | Host for protein expression | GE Healthcare |
| Y. enterocolitica | ||
| WA-314 | Y. enterocolitica serotype O:8 expressing YadA | [27] |
| WA-C | Plasmidless derivative of WA-314, YadA− | [27] |
| Plasmid | ||
| pUC118 | Cloning vector, Apr | Takara |
| pCR-XL-TOPO | Cloning vector, Kmr | Invtirogen |
| pDONR221 | Donor vector for Gateway technology, Kmr | Invtirogen |
| pGEX4T-2 | Expression vector for GST fusion protein, Apr | GE healthcare |
| pWH1266 | E. coli-Acinetobacter shuttle plasmid, Tcr, Apr | [47] |
| pJQ200sk | Mobile plasmid, SacB, Gmr | [46] |
| pP3 | Source of P3-Apr cassette; high resistance to ampicilin, Kmr, Apr | T. Kurihara |
| pSAE1 | pJQ200sk::Acinetobacter origin, SacB, Gmr | This study |
| pSAE2 | pSAE::PvuII site, ΔSacB, Gmr | This study |
| pSAE3 | pSAE::P3-Apr, ΔSacB, Gmr, Apr | This study |
| pJN105 | Source of araC-PBAD system, Gmr | [48] |
| pARP3 | E. coli-Acinetobacter shuttle expression vector, pSAEΔSacB::P3 Apr::araC-PBAD, Gmr, Apr | This study |
| pAtaA | ataA-expression vector, pARP3::ataA | This study |
a
Ap, ampicillin; Km, kanamycin; Tc, tetracycline; Gm, gentamycin.