Figure 3. Cell-type specific expression of EGFP two weeks after injection of the 2.0 kbpVPI.EGFP rAAV in the SON.
The endogenous fluorescence of the expressed EGFP (A, D) colocalizes with cells that are labeled by a vasopressin-neurophysin specific monoclonal antibody marker, PS41 (B), as seen in a merge of EGFP fluorescence and PS41 immunoreactivity (C), but does not colocalize with cells labeled with an oxytocin-neurophysin specific monoclonal antibody marker, (PS38) (E), as shown in the merged image in panel F. 100 µm scale bar in panel F is the same for all images. Abbreviation: OC, optic chiasm.