Table 1. Sampling localities and Genbank accession numbers from individuals of Cambarellus used in this study.
| + | Species id fromthis study | Subgenus(Fitzpatrick,1983) | GeneBank accession numbers | ||||
| 16S | 12S | cox1 | 28S | H3 | |||
| 1 | Cambarellus blacki | Pandicambarus | JX127836 | JX127697 | JX127977 | JX127568 | JX127429 |
| 1 | Cambarellus blacki | Pandicambarus | JX127837 | JX127698 | JX127978 | JX127569 | JX127430 |
| 2 | Cambarellus diminutus * | Pandicambarus | JX127810 | JX127953 | JX127545 | JX127405 | |
| 3 | Cambarellus lesliei * | Pandicambarus | JX127809 | JX127952 | JX127544 | JX127404 | |
| 4 | Cambarellus ninae | Pandicambarus | JX127814 | JX127957 | JX127549 | JX127409 | |
| 5 | Cambarellus ninae ** | Pandicambarus | JX127833 | JX127694 | JX127974 | JX127565 | JX127426 |
| 6 | Cambarellus puer1233 | Pandicambarus | JX127822 | JX127686 | JX127965 | JX127557 | JX127417 |
| 7 | Cambarellus puer | Pandicambarus | JX127815 | JX127958 | JX127550 | JX127410 | |
| 8 | Cambarellus schmitti | Pandicambarus | JX127811 | JX127954 | JX127546 | JX127406 | |
| 9 | Cambarellus schmitti | Pandicambarus | JX127838- | JX127699- | JX127979- | JX127570- | JX127431- |
| JX127855 | JX127714 | JX127996 | JX127587 | JX127447 | |||
| 10 | Cambarellus schmitti | Pandicambarus | JX127856 | JX127715 | JX127997 | JX127448 | |
| 11 | Cambarellus texanus | Pandicambarus | JX127832 | ||||
| 12 | Cambarellus texanus *** | Pandicambarus | JX127834 | JX127695 | JX127975 | JX127566 | JX127427 |
| 13 | Cambarellus texanus | Pandicambarus | JX127819 - | JX127683 – | JX127962 – | JX127554 – | JX127414 – |
| JX127821 | JX127685 | JX127964 | JX127556 | JX127416 | |||
| 14 | Cambarellus shufeldtii | Dirigicambarus | JX127812 | JX127955 | JX127547 | JX127407 | |
| 15 | Cambarellus shufeldtii | Dirigicambarus | JX127816 | JX127959 | JX127551 | JX127411 | |
| 16 | Cambarellus shufeldtii | Dirigicambarus | JX127817 | JX127960 | JX127552 | JX127412 | |
| 17 | Cambarellus shufeldtii | Dirigicambarus | |||||
| 18 | Cambarellus shufeldtii | Dirigicambarus | JX127835 | JX127696 | JX127976 | JX127567 | JX127428 |
| 19 | Cambarellus shufeldtii | Dirigicambarus | JX127857 | JX127998 | JX127588 | JX127449 | |
| 20 | Cambarellus shufeldtii | Dirigicambarus | JX127818 | JX127961 | JX127553 | JX127413 | |
| 21 | Cambarellus zempoalensis | Cambarellus | JX127725 | JX127599 | JX127868 | JX127460 | JX127320 |
| 21 | Cambarellus zempoalensis | Cambarellus | JX127770 | JX127644 | JX127913 | JX127505 | JX127365 |
| 22 | Cambarellus zempoalensis | Cambarellus | JX127747 | JX127621 | JX127890 | JX127482 | JX127342 |
| 22 | Cambarellus zempoalensis | Cambarellus | JX127759 | JX127633 | JX127902 | JX12749 | JX127354 |
| 22 | Cambarellus zempoalensis | Cambarellus | JX127772 | JX127646 | JX127915 | JX127507 | JX127367 |
| 22 | Cambarellus zempoalensis | Cambarellus | JX127773 | JX127647 | JX127916 | JX127508 | JX127368 |
| 23 | Cambarellus zempoalensis | Cambarellus | JX127750 | JX127624 | JX127893 | JX127485 | JX127345 |
| 23 | Cambarellus zempoalensis | Cambarellus | JX127756 | JX127630 | JX127899 | JX127491 | JX127351 |
| 24 | Cambarellus zempoalensis | Cambarellus | JX127786 | JX127660 | JX127929 | JX127521 | JX127381 |
| 25 | Cambarellus zempoalensis | Cambarellus | JX127744 | JX127618 | JX127887 | JX127479, | JX127339 |
| 25 | Cambarellus zempoalensis | Cambarellus | JX127753 | JX127627 | JX127896 | JX127488 | JX127348 |
| 25 | Cambarellus zempoalensis | Cambarellus | JX127794 | JX127668 | JX127937 | JX127529 | JX127389 |
| 26 | Cambarellus zempoalensis | Cambarellus | JX127743 | JX127617 | JX127886 | JX127478 | JX127338 |
| 26 | Cambarellus zempoalensis | Cambarellus | JX127755 | JX127629 | JX127898 | JX127490 | JX127350 |
| 26 | Cambarellus zempoalensis | Cambarellus | JX127765 | JX127639 | JX127908 | JX127500 | JX127360 |
| 26 | Cambarellus zempoalensis | Cambarellus | JX127791 | JX127665 | JX127934 | JX127526 | JX127386 |
| 27 | Cambarellus zempoalensis | Cambarellus | JX127732 | JX127606 | JX127875 | JX127467 | JX127327 |
| 28 | Cambarellus zempoalensis | Cambarellus | JX127771 | JX127645, | JX127914 | JX127506 | JX127366 |
| 28 | Cambarellus zempoalensis | Cambarellus | JX127793 | JX127667 | JX127936 | JX127528 | JX127388 |
| 29 | Cambarellus zempoalensis | Cambarellus | JX127736 | JX127610 | JX127879 | JX127471 | JX127331 |
| 29 | Cambarellus zempoalensis | Cambarellus | JX127754 | JX127628 | JX127897 | JX127489 | JX127349 |
| 29 | Cambarellus zempoalensis | Cambarellus | JX127789 | JX127663 | JX127932 | JX127524 | JX127384 |
| 29 | Cambarellus zempoalensis | Cambarellus | JX127805 | JX127679 | JX127948 | JX127540 | JX127400 |
| 30 | Cambarellus zempoalensis | Cambarellus | JX127798 | JX127672 | JX127941 | JX127533 | JX127393 |
| 30 | Cambarellus zempoalensis | Cambarellus | JX127799 | JX127673 | JX127942 | JX127534 | JX127394 |
| 31 | Cambarellus patzcuarensis | Cambarellus | JX127728 | JX127602 | JX127871 | JX127463 | JX127323 |
| 31 | Cambarellus patzcuarensis | Cambarellus | JX127740 | JX127614 | JX127883 | JX127475 | JX127335 |
| 31 | Cambarellus patzcuarensis | Cambarellus | JX127751 | JX127625 | JX127894 | JX127486 | JX127346 |
| 31 | Cambarellus patzcuarensis | Cambarellus | JX127774 | JX127648 | JX127917 | JX127509 | JX127369 |
| 32 | Cambarellus patzcuarensis | Cambarellus | JX127802 | JX127676 | JX127945 | JX127537 | JX127397 |
| 32 | Cambarellus patzcuarensis | Cambarellus | JX127803 | JX127677 | JX127946 | JX127538 | JX127398 |
| 33 | Cambarellus patzcuarensis | Cambarellus | JX127741 | JX127615 | JX127884 | JX127476 | JX127336 |
| 33 | Cambarellus patzcuarensis | Cambarellus | JX127775 | JX127649 | JX127918 | JX127510 | JX127370 |
| 34 | Cambarellus patzcuarensis | Cambarellus | JX127779 | JX127653 | JX127922 | JX127514 | JX127374 |
| 35 | Cambarellus sp. (cladeIII) | Cambarellus | JX127738 | JX127612 | JX127881 | JX127473 | JX127333 |
| 35 | Cambarellus sp. (cladeIII) | Cambarellus | JX127752 | JX127626 | JX127895 | JX127487 | JX127347 |
| 36 | Cambarellus chapalanus | Cambarellus | JX127726 | JX127600 | JX127869 | JX127461 | JX127321 |
| 36 | Cambarellus chapalanus | Cambarellus | JX127760 | JX127634 | JX127903 | JX127495 | JX127355 |
| 37 | Cambarellus chapalanus | Cambarellus | JX127733 | JX127607 | JX127876 | JX127468 | JX127328 |
| 37 | Cambarellus chapalanus | Cambarellus | JX127734 | JX127608 | JX127877 | JX127469 | JX127329 |
| 37 | Cambarellus chapalanus | Cambarellus | JX127737 | JX127611 | JX127880 | JX127472 | JX127332 |
| 37 | Cambarellus chapalanus | Cambarellus | JX127764 | JX127638 | JX127907 | JX127499 | JX127359 |
| 37 | Cambarellus chapalanus | Cambarellus | JX127830 | JX127693 | JX127972 | JX127564 | JX127425 |
| 38 | Cambarellus chapalanus | Cambarellus | JX127795 | JX127669 | JX127938 | JX127530 | JX127390 |
| 38 | Cambarellus chapalanus | Cambarellus | JX127796 | JX127670 | JX127939 | JX127531 | JX127391 |
| 38 | Cambarellus chapalanus | Cambarellus | JX127800 | JX127674 | JX127943 | JX127535 | JX127395 |
| 39 | Cambarellus chapalanus | Cambarellus | JX127742 | JX127616 | JX127885 | JX127477 | JX127337 |
| 39 | Cambarellus chapalanus | Cambarellus | JX127766 | JX127640 | JX127909 | JX127501 | JX127361 |
| 40 | Cambarellus chapalanus | Cambarellus | JX127783 | JX127657 | JX127926 | JX127518 | JX127378 |
| 41 | Cambarellus chapalanus | Cambarellus | JX127748 | JX127622 | JX127891 | JX127483 | JX127343 |
| 41 | Cambarellus chapalanus | Cambarellus | JX127749 | JX127623 | JX127892 | JX127484 | JX127344 |
| 41 | Cambarellus chapalanus | Cambarellus | JX127768 | JX127642 | JX127911 | JX127503 | JX127363 |
| 41 | Cambarellus chapalanus | Cambarellus | JX127792 | JX127666 | JX127935 | JX127527 | JX127387 |
| 41 | Cambarellus chapalanus | Cambarellus | JX127797 | JX127671 | JX127940 | JX127532 | JX127392 |
| 42 | Cambarellus chapalanus | Cambarellus | JX127781 | JX127655 | JX127924 | JX127516 | JX127376 |
| 43 | Cambarellus prolixus | Cambarellus | JX127729 | JX127603 | JX127872 | JX127464 | JX127324 |
| 43 | Cambarellus prolixus | Cambarellus | JX127761 | JX127635 | JX127904 | JX127496 | JX127356 |
| 43 | Cambarellus prolixus | Cambarellus | JX127762 | JX127636 | JX127905 | JX127497 | JX127357 |
| 44 | Cambarellus chapalanus | Cambarellus | JX127735 | JX127609 | JX127878 | JX127470 | JX127330 |
| 44 | Cambarellus chapalanus | Cambarellus | JX127769 | JX127643 | JX127912 | JX127504 | JX127364 |
| 45 | Cambarellus chapalanus | Cambarellus | JX127801 | JX127675 | JX127944 | JX127536 | JX127396 |
| 46 | Cambarellus chapalanus | Cambarellus | JX127739 | JX127613 | JX127882 | JX127474 | JX127334 |
| 46 | Cambarellus chapalanus | Cambarellus | JX127758 | JX127632 | JX127901 | JX127493 | JX127353 |
| 47 | Cambarellus chapalanus | Cambarellus | JX127745 | JX127619 | JX127888 | JX127480 | JX127340 |
| 47 | Cambarellus chapalanus | Cambarellus | JX127746 | JX127620 | JX127889 | JX127481 | JX127341 |
| 48 | Cambarellus prolixus | Cambarellus | JX127806 | JX127680 | JX127949 | JX127541 | JX127401 |
| 48 | Cambarellus prolixus | Cambarellus | JX127807 | JX127681 | JX127950 | JX127542 | JX127402 |
| 49 | Cambarellus prolixus | Cambarellus | JX127808 | JX127682 | JX127951 | JX127543 | JX127403 |
| 50 | Cambarellus chapalanus | Cambarellus | JX127804 | JX127678 | JX127947 | JX127539 | JX127399 |
| 51 | Cambarellus sp. (clade V) | Cambarellus | JX127780 | JX127654 | JX127923 | JX127515 | JX127375 |
| 52 | Cambarellus sp. (clade V) | Cambarellus | JX127782 | JX127656 | JX127925 | JX127517 | JX127377 |
| 53 | Cambarellus sp. (clade V) | Cambarellus | JX127787 | JX127661 | JX127930 | JX127522, | JX127382 |
| 53 | Cambarellus sp. (clade V) | Cambarellus | JX127788 | JX127662 | JX127931 | JX127523 | JX127383 |
| 54 | Cambarellus sp. (clade VI) | Cambarellus | JX127730 | JX127604 | JX127873 | JX127465 | JX127325 |
| 54 | Cambarellus sp. (clade VI) | Cambarellus | JX127757 | JX127631 | JX127900 | JX127492 | JX127352 |
| 54 | Cambarellus sp. (clade VI) | Cambarellus | JX127790 | JX127664 | JX127933 | JX127525 | JX127385 |
| 55 | Cambarellus montezumae | Cambarellus | JX127731 | JX127605 | JX127874 | JX127466 | JX127326 |
| 55 | Cambarellus montezumae | Cambarellus | JX127763 | JX127637 | JX127906 | JX127498 | JX127358 |
| 56 | Cambarellus montezumae | Cambarellus | JX127776 | JX127650 | JX127919 | JX127511 | JX127371 |
| 56 | Cambarellus montezumae | Cambarellus | JX127777 | JX127651 | JX127920 | JX127512 | JX127372 |
| 56 | Cambarellus montezumae | Cambarellus | JX127778 | JX127652 | JX127921 | JX127513 | JX127373 |
| 57 | Cambarellus sp. (clade VIII) | Cambarellus | JX127727 | JX127601 | JX127870 | JX127462 | JX127322 |
| 57 | Cambarellus sp. (clade VIII) | Cambarellus | JX127767 | JX127641 | JX127910 | JX127502 | JX127362 |
| 58 | Cambarellus occidentalis | Cambarellus | JX127784 | JX127658 | JX127927 | JX127519 | JX127379 |
| 58 | Cambarellus occidentalis | Cambarellus | JX127785 | JX127659 | JX127928 | JX127520 | JX127380 |
| 59 | Cambarellus occidentalis | Cambarellus | JX127813 | JX127956 | JX127548 | JX127408 | |
| Procambarus toltecae | JX127823 | JX127687 | JX127966 | JX127558 | JX127418 | ||
| Procambarus acutus1 | JX127824 | JX127688 | JX127967 | JX127559 | JX127419 | ||
| Procambarus acutus2 | JX127827 | JX127970 | JX127562 | JX127422 | |||
| Procambarus llamasi1 | JX127825 | JX127689 | JX127968 | JX127560 | JX127420 | ||
| Procambarus llamasi2 | JX127826 | JX127690 | JX127969 | JX127561 | JX127421 | ||
| Procambarus clarkii | JX127829 | JX127692 | JX127971 | JX127563 | JX127424 | ||
| Procambarus bouvieri | JX127828 | JX127691 | JX127423 | ||||
| Orconectes deanae | JX127859 | JX127717 | JX128000 | JX127590 | JX127451 | ||
| Orconectes ronaldi | JX127865 | JX127722 | JX128005 | JX127596 | JX127457 | ||
| Orconectes virilis1 | JX127866 | JX127723 | JX128006 | JX127597 | JX127458 | ||
| Orconectes virilis2 | JX127860 | JX127591 | JX127452 | ||||
| Cambarus brachydactylus ++ | DQ411732 | DQ411729 | DQ411783 | DQ411802 | |||
| Cambarus maculatus | JX127864 | JX127721 | JX128004 | JX127595 | JX127456 | ||
| Cambarus pyronotus | JX127862 | JX127719 | JX128002 | JX127593 | JX127454 | ||
| Cambarus striatus | JX127861 | JX127718 | JX128001 | JX127592 | JX127453 | ||
| Fallicambarus byersi | JX127863 | JX127720 | JX128003 | JX127594 | JX127455 | ||
| Fallicambarus caesius | JX127867 | JX127724 | JX128007 | JX127598 | JX127459 | ||
| Fallicambarus fodiens | JX127858 | JX127716 | JX127999 | JX127589 | JX127450 | ||
+
Locality number, as depicted in Figure 1.
*
Type specimens or type localities.
**
Morphologically identified as C. shufeldtii.
***
Morphologically identified as C. puer.
++
Sequence from the study of Buhay et al. 2007, tissue originally from the Carnegie Museum of Natural History.
Populations termed as ‘C. sp’ are new proposed taxa, according to phylogenetic structure (see Figure 3).
Populations from clade I are included in the lineage of C. zempoalensis, species which has to be re-examined by incorporing C. montezumae lermensis in the analysis.