Table 1. Tested classical protocols for the isolation of C. bombi from the host (after [38]).

Method	Procedure	Finding
Standard	Sample in medium, with addition of 2% Mäser mix. Incubation at 27°C, 3% CO2 for 24 h. Extraction of supernatant from 15 ml falcon tubes with 2–3 ml of medium.	C. bombi is enriched, but contaminated with yeast.
Density-Gradient	Stepwise gradient of 30%, 50%, 70% percoll in tubes containing medium. Extraction after centrifugation with respective speeds.	In all cases, no distinct layer of C. bombi could be extracted.
Serum-Gradient	In medium, with addition of 25% serum albumin, and a 10% layer on top. After centrifugation, tube cultured for 24 h (27°C, 3% CO2).	C. bombi enriched at boundary layer between serum gradients. Extractions from this layer free of yeast but difficult to perform.