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. 2012 Nov 14;7(11):e48299. doi: 10.1371/journal.pone.0048299

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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A) Immunohistochemistry was used to detect the expression of EGFR (a), ErbB2 (b), ErbB3 (c) and ErbB4 (d) in paraffin-embedded human GCT tissues. Magnification, 400×. B) Western blot detection of expression of ErbB family receptor proteins in human ovarian granulosa cells and GCT cell lines. SKOV-3 and COV644 cells were used as controls for the detection system. C) Fluorescent immunohistochemistry localization of ErbB receptors in KGN cells. The images were captured with a confocal laser scanning microscope. Arrows indicate the membrane localization of ErbBs in KGN cells. Magnification, 630×; Ab-control: 2^nd antibody only control, magnification, 200×.