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. 2012 Nov 14;7(11):e48874. doi: 10.1371/journal.pone.0048874

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© 2012 Jiang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a–c Triglyceride (TG) levels were measured in differentiating adipocytes after incubation with LA (L) or t10c12 CLA (C), with or without the SIRT1 activator SRT1720 (SRT), or the sirtuin inhibitors sirtinol (SOL), nicotinamide (NAM), or etomoxir (ETO) for 24 h. d Immunoblot analysis of whole cell extracts for the amount of SIRT1 and β-actin proteins after exposure to 80 nmol/L of control siRNA (siCON), or siRNA against SIRT1 (siSirt1), or transfection reagent only. Duplicate loadings of each sample were analyzed and the lanes marked by 1/3 have one third of the indicated samples loaded. e Triglyceride (TG) amounts after treatments as in d. Each bar in panels a–c and e represents the mean + SEM (n = 3), and is representative of three independent experiments. Means not sharing a common letter differ, P 0.05. Panel d is a representative blot of three independent experiments.

Inline graphic — a–c Triglyceride (TG) levels were measured in differentiating adipocytes after incubation with LA (L) or t10c12 CLA (C), with or without the SIRT1 activator SRT1720 (SRT), or the sirtuin inhibitors sirtinol (SOL), nicotinamide (NAM), or etomoxir (ETO) for 24 h. d Immunoblot analysis of whole cell extracts for the amount of SIRT1 and β-actin proteins after exposure to 80 nmol/L of control siRNA (siCON), or siRNA against SIRT1 (siSirt1), or transfection reagent only. Duplicate loadings of each sample were analyzed and the lanes marked by 1/3 have one third of the indicated samples loaded. e Triglyceride (TG) amounts after treatments as in d. Each bar in panels a–c and e represents the mean + SEM (n = 3), and is representative of three independent experiments. Means not sharing a common letter differ, P 0.05. Panel d is a representative blot of three independent experiments.