Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Nov 14;7(11):e49232. doi: 10.1371/journal.pone.0049232

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

(A) Analysis of PKCδ protein levels in SF- and SR-HsFb with or without PMA treatment. PKCδ protein level was not different between SF- and SR-cells. (B) SF-HsFb were transfected with PKCδ siRNA or a control scRNA. PKCδ expression in the transfected cells was analyzed by Western blotting. (C) SF-HsFb (upper panel) and SR-HsFb (lower panel) were transfected with PKCδ siRNA or scRNA. PMA-induced COX-2 expression was analyzed by Western blotting. The blots were quantified by densitometry (mean ± SEM, n = 3). (D) PKCδ activity in SF- vs. SR-HsFb treated with PMA was determined by immunoprecipitation (IP) to isolate PKCδ proteins and analyzed the PKC catalytic activity of the IP-isolated PKCδ.