Skip to main content
. 2012 Nov 14;7(11):e48963. doi: 10.1371/journal.pone.0048963

Figure 1. Quantification of CYP11A1 mRNA abundance in normal and PCOS theca cells.

Figure 1

The time course of CYP11A1 mRNA accumulation was examined in A) normal or B) PCOS theca cells treated in serum free medium for 0, 4, 8, 16, 24, or 48 h in the presence and absence of 20 µM forskolin. C) CYP11A1 mRNA accumulation in normal and PCOS theca cells following 24 h treatment with and without 20 µM forskolin serum free medium. CYP11A1 mRNA was measured using quantitative real-time PCR as described in Materials and Methods. The data presented in Panel A and Panel B are data obtained from two normal and two PCOS patients, that are representative of data collected from theca cells from 5 normal and 5 PCOS patients. The data presented in Panel C are the results from independent analyses of theca cells isolated from 5 normal and 5 PCOS women. CYP11A1 mRNA accumulation was increased in PCOS theca cells as compared to normal theca cells under both control (a, P<0.01) and forskolin-stimulated conditions (b, P<0.01). Forskolin- treatment significantly increased CYP11A1 mRNA accumulation in both normal and PCOS theca cells (*, P<0.01).