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. 2012 Nov 14;7(11):e48963. doi: 10.1371/journal.pone.0048963

Figure 2. Deletion analysis of the CYP11A1 promoter in normal and PCOS theca cells.

Figure 2

A) Theca cells were transiently transfected with pGL3 luciferase constructs containing −2327, −1676, −660, −160, or −90 to +49 bp of the 5′-flanking sequence of the CYP11A1 gene. All constructs contain the endogenous TATA box and transcriptional start site. B) Normal and PCOS theca cells were transiently transfected with the above constructs described in Materials and Methods. Following transfection, cells were cultured in transfection medium alone or with forskolin (20 µM) for 48 h. Data are presented as relative luciferase (LUC) activity that was normalized with β-galactosidase activity, and represent the mean ± SEM of independent experiments in five normal and five PCOS theca cell cultures. CYP11A1 promoter activity was increased in PCOS theca cells, under basal (a, P<0.01) and forskolin-stimulated conditions (b, P<0.01), as compared to normal theca cells for individual promoter constructs.