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. 2012 Nov 14;7(11):e48963. doi: 10.1371/journal.pone.0048963

Figure 5. Endogenous CYP11A1 mRNA half-life in normal and PCOS theca cells.

Figure 5

The stability of endogenous CYP11A1 mRNA was examined in normal and PCOS theca cells under untreated and forskolin (20 µM)-stimulated conditions following treatment with the transcriptional inhibitor DRB (75 µM) from 2–42 h. A) Graphical representation of the amount of CYP11A1 mRNA remaining at each time interval, determined by quantitative real-time PCR. B) The half-life of endogenous CYP11A1 mRNA is presented as the mean ± SEM from independent examinations in 5 normal and 5 PCOS theca cell cultures. CYP11A1 mRNA half-life was increased in PCOS theca, under both untreated (a, P<0.01) and forskolin-stimulated conditions (a, P<0.01). Forskolin treatment did not significantly alter CYP11A1 mRNA half-life in normal or PCOS theca cells.