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. 2012 Nov 14;7(11):e48875. doi: 10.1371/journal.pone.0048875

Figure 2. Toll-like Receptor Transmembrane Domain Homotypic Interactions.

Figure 2

(A) The ToxR assay was used to study homotypic interactions of the TLR TMDs. A chimeric protein expressing the TMD of interest was monitored through β-galactosidase activity. In all cases, we saw that the TLRs have interaction potential solely from TMD-TMD interactions. Each TLR TMD interaction measurement analysis was performed on 3 technical replicates with >6 measurements for each replicate. Error bars depict the standard error of the mean (n – Table S1). Western blots staining for MBP were performed to monitor expression levels of the constructs – (B) chimeric maltose binding protein expression, (C) endogenous maltose binding protein expression. All samples were normalized to the GpA signal and expression levels. Significant differences were determined by use of the Tukey-Kramer test with all TLRs being significantly different than the negative control.