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. 2012 Nov 14;7(11):e48875. doi: 10.1371/journal.pone.0048875

Figure 7. Toll-like Receptor 2 Interaction Interface Studied by Mutagenesis.

Figure 7

TLR2 residues were mutated to study effects on homotypic and heterotypic interactions using the ToxR assay. These mutations were at positions identified in the sequence as likely interaction locations. (A) Homotypic interactions of point mutations show no difference in interaction potential from wild-type TLR2 TMD. (B) Heterotypic interactions of point mutations show the potential importance of both the Ala597 and Cys609 positions for heterotypic interactions. Western blots staining for MBP were performed to monitor expression levels of the constructs with the upper band being functional ToxR chimeras and the lower band being nonfunctional ToxR* chimeras – (C) TLR2, (D) TLR2 G593V, (E) TLR2 A597V, and (F) TLR2 C609I.