Figure 1.

Induction of extracellular alkalinization in suspension-cultured tomato cells in response to two consecutive stimuli. Solid lines, Extracellular pH in untreated cells (control) or cells treated with CH4 or ergosterol at the concentrations indicated. Shaded circles and dotted lines, Extracellular pH after a second stimulation with 10 nm CH4 (shown in the inset). The pH of the growth medium was 5.1 at the start of the experiment.