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. Author manuscript; available in PMC: 2013 Oct 16.
Published in final edited form as: Anal Chem. 2012 Oct 5;84(20):8505–8513. doi: 10.1021/ac301091m

Figure 1.

Figure 1

Effect of capillary length on SPME-CE-MS/MS separation. The electropherograms of one step elution and CE separation of 16 protein mixture tryptic digests using (A) 90 cm and (C) 190 cm. The extracted ion electropherograms of 3 representative ions are shown from both (B) 90 cm and (D) 190 cm capillary lengths. Run conditions: 95 mM HOAc - 5% MeOH (pH 3.1) was used as the background electrolyte. Peptides were eluted with 100 nL 95% MeOH - 50 mM NH4OAc, pH 4.5. 30 kV was applied at the inlet of both 90 cm and 190 cm capillaries. MS/MS acquisition began upon application of separation voltage.