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. Author manuscript; available in PMC: 2013 Oct 16.
Published in final edited form as: Anal Chem. 2012 Oct 5;84(20):8505–8513. doi: 10.1021/ac301091m

Figure 2.

Figure 2

Effect of elution buffer on the elution efficiency, peptide identifications, and protein sequence coverages. The 16 protein mixture tryptic digests were eluted hydrodynamically with 100 nL elution volumes of 3 different buffers (95% MeOH, 85% MeOH - 10% isopropanol (IPA) and 48% MeOH - 48% IPA), followed by CE-MS/MS. An equal amount of protein was directly injected onto the capillary without SPME to simulate an elution efficiency of 100% for comparison. Peptide identifications and the average protein sequence coverage from triplicate analyses are plotted. 82×74mm (300 × 300 DPI)