Components for prokaryote method | Amount (μl) | Final |
| ||
pMALc-H (100 μg ml−1) | 5 | 0.5 μg |
BSA (1 mg mL−1) | 3 | 100 μg ml−1 |
NEB buffer 2 (10×) | 3 | 1× |
BamHI (20,000 U ml−1) | 1 | 10 U |
HindIII (20,000 U ml−1) | 1 | 10 U |
H20 | 17 |
Components for prokaryote method | Amount (μl) | Final |
| ||
pMALc-H (100 μg ml−1) | 5 | 0.5 μg |
BSA (1 mg mL−1) | 3 | 100 μg ml−1 |
NEB buffer 2 (10×) | 3 | 1× |
BamHI (20,000 U ml−1) | 1 | 10 U |
HindIII (20,000 U ml−1) | 1 | 10 U |
H20 | 17 |