| Instrument | GE Healthcare ÄKTA purifier |
| Flow rate | 0.8 ml min−1 |
| Column | GE Healthcare Superdex 200 10/300 GL |
| Column temperature | 4 °C |
| Particle size | 13 μm |
| Binding/elution buffer | PBS, pH 7.0 |
| Injection volume | 0.5 ml |
| Elution fraction size | 0.5 ml |
| Elution volume | 1.5 column volumes |
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