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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Nat Protoc. 2012 May 10;7(6):1052–1067. doi: 10.1038/nprot.2012.045
Mobile phase A: 0.1% (wt/vol) TFA in H2O
B: 0.1% (wt/vol) TFA in ACN
Flow rate 0.2 ml min−1
Columns Phenomenex Jupiter Column Proteo 90A (4 μm, 90 Å, 250 × 2.0 mm)
Column temperature 37 °C
Autosampler temperature 5 ° ± 3 °C
Injection volume 100 μl
Detector wavelength 214 nm
Run time 90 min