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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Nat Protoc. 2012 May 10;7(6):1052–1067. doi: 10.1038/nprot.2012.045
Ionization Electrospray positive, V Mode
Scan type MS scan
Mass range 100–4,000
Capillary voltage 3.0 kV
Sampling cone voltage 25.0 V
Source temperature 110 °C
Desolvation temperature 300 °C
Cone gas flow 40.0 liters h−1
Desolvation gas flow 800 liters h−1
LM resolution 4.7 V
HM resolution 15.0 V
Collision cell entrance 2.0 V
Collision exit −10.0 V
Collision gas flow 0.35 liters per h
Reference scan frequency 10 s