| Components for eukaryote method | Amount (μl) | Final |
|
| ||
| pFuse-mFc2 (100 μg ml−1) | 5 | 0.5 μg |
| BSA (1 mg mL−1) | 3 | 100 μg ml−1 |
| NEB buffer 3 (10×) | 3 | 1× |
| BgLII (20,000 U ml−1) | 1 | 10 U |
| EcoRV (20,000 U ml−1) | 1 | 10 U |
| H2O | 17 | |
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