| Component | Amount (μl) | Final |
|
| ||
| 5× Buffer | 10.0 | 1× |
| pMAL-c4E (100 μg ml−1) | 1.0 | 100 ng |
| Oligonucleotide 5 (5 mM) | 5.0 | 500 μM |
| Oligonucleotide 6 (5 mM) | 5.0 | 500 μM |
| dNTP (2.5 mM/base) | 5.0 | 250 μM/base |
| Nuclease-free H20 | 20.5 | |
| DMSO | 2.5 | 5.0% (vol/vol) |
| Phusion hot start (2 U μl−1) | 1.0 | 2 U |
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