Figure 2.

Effect of anthocyanins on secA transcription. H. pylori was cultured with 100 μM of anthocyanin in Mueller-Hinton broth/10% FBS for 3 days. After extensive washing, total RNA was extracted and cDNA synthesized. RT-PCR analysis was performed to assess expression of secA, cagA, and vacA. The galE (UDP-galactose 4-epimerase gene) was used as an internal control. No RT, no reverse transcriptase. A representative image from five independent experiments.