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. 2012 Nov 7;9(10):881–893. doi: 10.7150/ijms.5011

Figure 2.

Figure 2

Cell cycle distribution and expression of cell cycle regulator in TAMR/MCF-7 cells with or without drug treatment. (A) TAMR/MCF-7 cells were treated with SAHA at the indicated concentrations for 48 h. Cells stained with propidium iodine (PI) were subjected to flow cytometry analysis to determine the distribution at each phase of the cell cycle. (B) TAMR/MCF-7 cells were treated with SAHA at the indicated concentrations for 48 h. The TAMR/MCF-7 cells were homogenized, and the proteins were isolated. Aliquots of proteins were immunoblotted with specific primary antibodies against Cdc2, Cdk6, p27, cyclin A, cyclin B1, and cyclin D1. Equal loading and transfer were reproved the membranes with β-actin antibody.