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. 2012 Jul 24;20(11):2019–2030. doi: 10.1038/mt.2012.134

Figure 4.

Figure 4

Post-mortem assessment of retinal morphology in dog A5 at 4 months postinjection. (a–c) Nasal retinal cryosections from the treated and untreated eyes of dog A5 at 4 months post subretinal delivery of rAAV2/5RK.cpde6β in the nasal superior retina. (a) Wide A5 retinal section displaying vector exposed and unexposed-areas. Retinal layers remained intact while the choroid has been partially detached from the retina during the embedding process. (b) Fundus photograph representing the A5 retina exposed to the rAAV2/5 vector (dark circle) and the localization of the wide retinal section (red line). (c) Nasal retinal cryosections from unaffected, untreated dog NA2 at 5 months of age and from the untreated and treated eyes of dog A5. Serial retinal cryosections were processed for hematoxylin and eosin coloration (top) and for immunohistochemistry using antibodies against rod PDE6 (middle) or GNAT1 (bottom). Primary antibodies were detected with Alexa 488-conjugated goat anti-rabbit IgG (green). Cell nuclei were counterstained with DAPI (blue). Bar = 10 µm. Vertical dark lines indicate ONL thickness (mean ± SEM, n = 10). GC, ganglion cells; INL, inner nuclear layer; IS, inner segments; ONL, outer nuclear layer; OS, outer segments; rAAV, recombinant adeno-associated virus; RPE, outer retinal pigment.