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Southern-blot analysis of the β-amy1 structural gene in different barley cultivars. Barley genomic DNA was digested with HindIII, fractionated by agarose-gel electrophoresis, Southern blotted, and hybridized with β-amy1 pcβC51 cDNA probe. The genomic DNAs were from cv Adorra (lane 1), line 86-H2-64 (lane 2), H. spontaneum PI 296897 (lane 3), and cv Haruna Nijo (lane 4).
