The β-amy1 gene organization.
Top, Schematic presentation of the β-amy structural
gene. The β-amy1 ORF is composed of seven exons (black
boxes) interrupted by six introns (numerals I–VI) located within a DNA
fragment of approximately 5 kb. ATG, TAG, and gray box
indicate the translational start and stop codons and the approximately
1.3-kb promoter, respectively. Bottom, Sequence alignment of the
5′-proximal ends of intron III from β-amy1 alleles of
H. spontaneum PI 296897 (2), cv Adorra (3), and cv
Haruna Nijo (4). Line 1 shows the amino acid sequence (residues
195–205) encoded by the DNA sequence upstream of intron III. The
sequences of intron III from the β-amy1 allele of line
86-H2-64 (not shown) and H. spontaneum PI 296897 (2) are
identical. The β-amy1 genomic sequences are in the
EMBL-GenBank database under accession numbers AF061204 (H.
spontaneum PI 296897), AF061203 (cv Adorra), and D49999 (cv
Haruna Nijo).