Table 1. PCR primers and annealing temperatures.

Target gene name	JGI IMG gene object ID	Primer sequences		Annealing temperature for PCR
		Forward	Reverse
cbiD	2014753801	5′-ACCGCCAGCCTCAGGGTTGA-3′	5′-ACAGCCGCCATGGCACACAG-3′	59 °C
cbiF	2014753804	5′-CGCTGTCTGGAAGAAGCCGACC-3′	5′-TGCATGGCGGAGGCCAGATT-3′	57 °C
cbiC	2014753814	5′-CGCCGTTGTCCGCCAGCTTA-3′	5′-TTTCACCCGCCGCTTCTGCC-3′	58 °C

Abbreviations: IMG, integrated microbial genomes; JGI, Joint Genome Institute.