Figure 1.

Estimates of alpha diversity with Bonferroni-corrected confidence bounds calculated with CatchAll parametric models for pooled sequences from duplicate samples (when available, see Supplementary Table S1) for (a) Bacteria and (b) Archaea, and calculated as the Chao2 index with paired duplicate samples for (c) Eukarya. For Bacteria and Archaea, sequences from epilimnion and hypolimnion samples were pooled by season for Toolik Lake summer and Toolik Lake winter. Diversity estimates were calculated for all sequences (closed symbols) and for reduced sequence data sets that were randomly resampled to equal sample size (open symbols).