Table 1. Quality and quantity of DNA extracted from the Rothamsted Parkgrass soil with different DNA extraction approaches.
Extraction method | Quantity of soil used (g) | Principal type of lysis | Average DNA length after extraction (kbp) | DNA yield per kilogram of soil |
---|---|---|---|---|
MP BIO1O1 rhisosphere soil | 0.5 | Mechanical | 10 | 40 mg |
MP BIO1O1 soil | 0.5 | Mechanical | 10 | 10 mg |
MoBIO soil | 0.5 | Mechanical | 10 | 2 mg |
MP BIO1O1 on extracted cells (Nycodenz) | 300–400 | Mechanical | 10 | 150 μg |
In plug on extracted cells (Nycodenz) | 300–400 | Chemical-enzymatic | >500 | 120 μg |
DNA tissue on extracted cells (Nycodenz) | 300–400 | Chemical-enzymatic | 20–40 | 30 μg |
Gram positive on extracted cells (Nycodenz) | 300–400 | Chemical-enzymatic | 20–40 | 5 μg |