Figure 1.

Delayed colonization of attlp mutants by P. indica. Three-week-old plants were inoculated with P. indica, and fungal biomass was determined during biotrophic (3 dai) and cell death-dependent (7 dai) colonization (A) or at 3, 5, 7, and 9 dai (B) by qRT-PCR. A, Clades indicate the membership of tested mutants. The relative amount of fungal biomass in mutant roots was related to the wild type (WT; set to 1). Data are averages from at least three independent experiments, and error bars represent se. B, This experiment was performed twice with similar results. se values are from three technical replicates of one biological experiment. For both experiments, 200 plants were analyzed per mutant or wild type and per time point. Asterisks indicate significance at P < 0.05 (*), P < 0.01 (**), or P < 0.001 (***) as analyzed by Student’s t test.