Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 27;188(11):5655–5664. doi: 10.4049/jimmunol.1102330

FIGURE 1.

FIGURE 1

Upregulation of PGD2 and arachidonic acid cascade enzymes during CLP-induced sepsis. Female BALB/c mice were subjected to sham or five-puncture CLP surgery. A and B, The peritoneal lavage fluid was collected at 20 h after the surgery. The level of PGD2 (A) and 15-deoxy-delta-12,14-PGJ2 (15d-PGJ2) (B) in the cell-free peritoneal lavage fluid was measured by ELISA. C and D, The peritoneal exudate cells were collected 4 and 20 h after the surgery. The mRNA expression of CRTH2 (Gpr44) (C) and DP1 (Ptgdr) (D) in the peritoneal exudate cells was measured by quantitative real time PCR (qRT-PCR). The gene expression of secretory phospholipase A2 (PLA2) group IIA (Pla2g2a) (E), cytosolic PLA2 group 4A (Pla2g4a) (F), COX-2 (Ptgs2) (G), COX-1 (Ptgs1) (H), L-PGDS (Ptgds) (I), and H-PGDS (Hpgds) (J) in the peritoneal exudate cells was measured by qRT-PCR. Data are expressed as mean ± SEM. n = 5–6 in each group. All data of qRT-PCR shown are ‘fold induction’ relative to 4 h after sham operation. *, p < 0.05, compared with sham operated group. NS, not significant. These results are representative of two or three different experiments.