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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 27;188(11):5655–5664. doi: 10.4049/jimmunol.1102330

FIGURE 2.

FIGURE 2

Improved survival and enhanced bacterial clearance in lethal sepsis induced by CLP in CRTH2−/− mice. A, WT (n = 12) and CRTH2−/− (n = 12) mice were subjected to five-puncture CLP surgery and survival was monitored for 96 h. B, WT (n = 25) and CRTH2−/− (n = 23) mice were subjected to seven-puncture CLP surgery and survival was monitored for 96 h. The data of (B) are the composite of three independent experiments. ***, p = 0.0001; **, p = 0.0002, compared with WT group. C-F, Peritoneal lavage fluid and blood was harvested at 6 and 24 h after five-puncture CLP surgery. Peritoneal lavage fluid and blood were serially diluted and plated on the Trypticase Soy Agar (TSA) plates. Bacteria levels at 6 h after CLP surgery in peritoneal lavage fluid (C) and blood (D), and at 24 h in peritoneal lavage fluid (E) and blood (F) were expressed as colony forming units (CFU) per 20 µl. These data are the composite of two independent experiments. The horizontal bar indicates the mean for each group. n = 10–12 in each group. *, p < 0.05, compared with WT mice.