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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 27;188(11):5655–5664. doi: 10.4049/jimmunol.1102330

FIGURE 3.

FIGURE 3

Altered cytokine levels and protective roles of IL-10 in CRTH2−/− mice after CLP surgery. A, At 6 and 20 h after five-puncture CLP surgery, peritoneal lavage fluid was collected and the level of TNF-α, IL-1β, IL-6, CCL3, IL-12p70, and IL-10 was measured by multiplexed immunobead-based assay. B, Serum was also collected at indicated time points and the level of TNF-α, IL-1β, IL-6, CCL3, IL-12p70, and IL-10 was measured by multiplexed immunobead-based assay. Data are expressed as mean ± SEM pg per ml of peritoneal lavage fluid or serum. n = 5–6 in each group. *, p < 0.05, compared with WT group. These results are representative of two different experiments. C, WT and CRTH2−/− mice received either control IgG (250 µg/mouse) or anti-IL-12 antibody (250 µg/mouse) 1 h before seven-puncture CLP surgery. The survival was monitored for 96 h. These data are the composite of two independent experiments. n = 9–10 mice in each group. D, WT and CRTH2−/− mice received either control IgG (250 µg/mouse) or anti-IL-10 antibody (250 µg/mouse) 1 h before seven-puncture CLP surgery. The survival was monitored for 96 h. These data are the composite of two independent experiments. n = 10 in each group. *, p < 0.05, compared with CRTH2−/− mice group which received control IgG.