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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Immunol. 2012 Apr 27;188(11):5655–5664. doi: 10.4049/jimmunol.1102330

FIGURE 6.

FIGURE 6

The roles of neutrophils and CXCR2 expression on the protection against CLP-induced sepsis in CRTH2−/− mice. A, WT and CRTH2−/− mice received either control IgG (250 µg/mouse) or anti-Gr-1 antibody (250 µg/mouse) 24 h before seven-puncture CLP surgery. The survival was monitored for 96 h. The data are the composite of two independent experiments. n = 10 in each group. *, p < 0.05. B-D, WT and CRTH2−/− mice received either control IgG (250 µg/mouse) or anti-Ly-6G antibody (250 µg/mouse) 24 h before seven-puncture CLP surgery. The survival was monitored for 96 h. n = 5 in each group. *, p < 0.05 (B). Peritoneal lavage fluids recovered at 24 h after CLP surgery from CRTH2−/− mice which were received either control IgG or anti-Ly-6G antibody were serially diluted and plated on the TSA plates. Bacteria levels were expressed as colony forming units (CFU) per 20 µl. The horizontal bar indicates the mean for each group. n = 7 in each group. *, p < 0.05, compared with control IgG-treated group (C). The levels of IL-10 in peritoneal lavage fluids recovered 24 h after CLP surgery were measured by ELISA. n = 5 in each group. *, p < 0.05 (D). E, WT and CRTH2−/− mice received either control DMSO or CXCR antagonist SB225002 (10 mg/kg body weight mouse) 1 h before seven-puncture CLP surgery. The survival was monitored for 96 h. The data are composite of two independent experiments. n = 10 in each group. *, p < 0.05.