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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Curr Opin Struct Biol. 2012 Mar 23;22(4):507–513. doi: 10.1016/j.sbi.2012.02.004

Figure 1.

Figure 1

Fluorescent methods used to explore a membrane protein structure. In the center is the crystal structure of the acid sensing ion channel ASICS1 (PDB 2QTS) positioned with its transmembrane domains in a membrane [61]. Surrounding the structure are example fluorescent methods including FRET between the two fluorescent dyes Cy3 and Cy5, transition metal ion FRET between the dye bimane and a di-histidine bound metal, photo-induced electron transfer (PET) between bimane and a tryptophan, and a tetramethyl-rhodamine fluorophore undergoing an environment-induced fluorescent change. The approximate distance scales that each technique works over are indicated by the surrounding arrows.