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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Protoc. 2012 Sep 27;7(10):1897–1908. doi: 10.1038/nprot.2012.103

Figure 3.

Figure 3

Precipitation of genomic DNA using the anti-CMS method. (a) For testing the efficiency of precipitation from genomic DNA, three sources with varying amounts of 5hmC were used. HEK293 cells contain little 5hmC, mouse ES cells contain intermediate levels and HEK293 cells expressing the TET1 catalytic domain (293 TET1) contain large amounts, as demonstrated by an anti-5hmC dot blot. (b) DNA from the HEK293, ES and HEK293 TET1 cells was sonicated, end-labeled and precipitated using the anti-CMS method. Note that the degree of pull-down in each sample correlates with the extent of hydroxymethylation observed in part a. Error bars show s.d. (n = 3). The data shown in this figure are reproduced with permission from ref. 27.