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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Protoc. 2012 Sep 27;7(10):1897–1908. doi: 10.1038/nprot.2012.103

TABLE 1.

Calculated 5hmC amount and IP efficiency per amplicon.

No. of 5hmC molecules per amplicon 81 20 4 2 1 0.5 0.25 0
5hmdCTP (%) 100 24.69 4.94 2.47 1.23 0.62 0.31 0
dCTP (%) 0 75.31 95.06 97.53 98.77 99.38 99.69 100
Theoretical IP efficiency 100 100 84.4 61.9 38.7 22.0 11.8 0