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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Protoc. 2012 Sep 27;7(10):1897–1908. doi: 10.1038/nprot.2012.103
Cycle Denature Anneal Extend Hold
1 95 °C, 2 min — — —
2 98 °C, 30 s
3–8 98 °C, 15 s 60 °C, 30 s 72 °C, 4 min —
9 — — 72 °C, 10 min —
10 — — — 4 °C, ∞