| Reagent | Volume (μl) | Final |
|---|---|---|
| DNA, 250 ng | X | 250 ng |
| T4 PNK (10 U μl−1) | 1 | 0.4 U |
| T4 PNK buffer, 10× | 2.5 | 1× |
| ATP [γ-32P] | 1 | |
| ddH2O | Fill to 25 |
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