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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Protoc. 2012 Sep 27;7(10):1897–1908. doi: 10.1038/nprot.2012.103
Reagent Volume (μl) Final
DNA sample 30 5 μg
T4 DNA ligase buffer with 10 mM ATP, 10× 10 1×
dNTP, 10 mM 4 0.4 mM
T4 DNA polymerase (3 U μl−1) 5 0.15 U
T4 Polynucleotide kinase (PNK; 10 U μl−1) 5 0.5 U
ddH2O Up to 100