| Reagent | Volume (μl) | Final |
|---|---|---|
| DNA sample | 32 | |
| Klenow buffer, 10× | 5 | 1× |
| dATP, 1 mM | 10 | 0.2 mM |
| Klenow exo - (5 U μl−1) | 3 | 0.33 U |
| ddH2O | Up to 50 |
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