| Reagent | Volume (μl) | Final |
|---|---|---|
| DNA sample | 10 | |
| DNA ligase buffer, 10× | 5 | 1× |
| PE methylated adapter mix | 10 | |
| T4 DNA ligase (400 U μl−1) | 1 | 8 U |
| ddH2O | Up to 50 |
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