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Temperature dependence of KIEs for ht-ADH mutants above the 30 °C break.
| Enzyme | [Ea(D) – Ea(H)], high kcal/mol |
Log(Aobs), lowc |
|---|---|---|
| WT | 0.2 (±0.2)b | 17.2 (±0.6) |
| L176V | 1.3 (±1.1) | 20.2 (±0.7) |
| L176A | 1.4(±0.9) | 19.6 (±0.2) |
| L176G | 1.2 (±1.0) | 20.6 (±0.8) |
| L176Δ | 1.7 (±0.4) | 25.2 (±1.6) |
| V260Aa | 1.9 (±1.0) | 24.1 (±1.0) |
The breakpoint for this mutant is at 40 °C.
Ea(T) – Ea(H); this parameter sets an upper limit on Ea(D) – Ea(H).
These data are for the protio-substrate (40).
