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. 2012 Jun 12;33(8):1572–1580. doi: 10.1093/carcin/bgs202

Fig. 5.

Fig. 5.

In vivo effect of p-DGA feeding on the proliferation and apoptosis markers as well as AR, PSA and CDKIs expression in 22Rv1 tumors. (A) IHC was performed in 22Rv1 xenograft tissues and immunoreactivity for PCNA, TUNEL and AR was quantified following the procedure described in the Materials and Methods section. Representative photographs are presented at 400×; insets represent further magnification of a part of the photographs. (B–C) Total cell lysates or nuclear and cytoplasmic fraction lysates were prepared from 22Rv1 xenograft tissues and analyzed for AR, PSA, p21 and p27 expression by western blotting. In each case, three random samples were analyzed from control and p-DGA treatment group (5mg/kg body weight). Membranes were reprobed with β-actin (total cell lysates), histone H1 (for nuclear fractions) or α-tubulin (for cytosolic fractions) to check protein loading. The densitometric values were normalized with respective loading control values (β-actin, histone H1 or α-tubulin) and presented as mean ± SEM.