FIG. 1.

Arsenic represses skeletal muscle and neuronal differentiation in P19 cells. P19 cells were aggregated as hanging drop cultures with 0, 0.1, 0.5, or 1.0μM sodium arsenite for 5 days, and then transferred to gelatin-coated plates. On day 7, myoblast-like cells were observed around the EBs, whereas on day 9, myoblasts, myotubes, and neurons could be identified. Arrows show myotubes and arrowheads indicate the differentiated neurons.